Publication detail

DNA isolation by superparamagnetic particles

CHROBOKOVÁ, M. RITTICH, B. ŠPANOVÁ, A. HORÁK, D.

Original Title

Type

lecture

Language

English

Original Abstract

In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become a powerful diagnostic tool for the identification of pathogens or beneficial microorganisms (probiotics) in foods. The isolation of DNA from analysed samples is always an integral part of a multistep identification procedure. High quality of isolated DNA (PCR-ready) is necessary for DNA amplification by PCR. Superparamagnetic particles have been attractive for molecular diagnostics applications due to their ability to interact with various biomolecules of interest. Manipulation with paramagnetic particles is easier than manipulation with non-magnetic ones. Reversible DNA adsorption on the surface of magnetic microparticles with carboxyl groups was used for the isolation of DNA. The lecture presents an overview of hydrophilic P(HEMA) based microspheres used as separation media for extraction and purification of DNAs in PCR-ready quality.

Keywords

Superparamagnetic particles, reversible DNA adsorption, DNA isolation in PCR-ready quality

Authors

CHROBOKOVÁ, M.; RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.

Released

22. 3. 2013

BibTex

@misc{BUT100456,
  author="Maria {Chroboková} and Alena {Španová} and Daniel {Horák} and Bohuslav {Rittich}",
  title="DNA isolation by superparamagnetic particles",
  year="2013",
  note="lecture"
}