Detail publikace

Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells

OLIVER, C. IBAÑEZ, R. FLORES-MERINO, M. VOJTOVÁ, L. ŠALPLACHTA, J. ČELKO, L. KAISER, J. MONTUFAR JIMENEZ, E.

Originální název

Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 mu m) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 mu m, d(50) = 65 mu m). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel.

Klíčová slova

polyvinylpyrrolidone; hydrogel; lyophilisation; nano-computed tomography; porosity; oral mucosa; stem cell

Autoři

OLIVER, C.; IBAÑEZ, R.; FLORES-MERINO, M.; VOJTOVÁ, L.; ŠALPLACHTA, J.; ČELKO, L.; KAISER, J.; MONTUFAR JIMENEZ, E.

Vydáno

5. 1. 2021

Nakladatel

MDPI

Místo

BASEL

ISSN

1996-1944

Periodikum

Materials

Ročník

14

Číslo

1

Stát

Švýcarská konfederace

Strany od

1

Strany do

14

Strany počet

14

URL

https://www.mdpi.com/1996-1944/14/1/227

Plný text v Digitální knihovně

http://hdl.handle.net/11012/195922

BibTex

@article{BUT168361,
  author="Carolina {Oliver} and Raúl Rosales {Ibañez} and Miriam {Flores-Merino} and Lucy {Vojtová} and Jakub {Šalplachta} and Ladislav {Čelko} and Jozef {Kaiser} and Edgar Benjamin {Montufar Jimenez}",
  title="Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells",
  journal="Materials",
  year="2021",
  volume="14",
  number="1",
  pages="1--14",
  doi="10.3390/ma14010227",
  issn="1996-1944",
  url="https://www.mdpi.com/1996-1944/14/1/227"
}