Detail publikace

An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry

ADAM, V. CHUDOBOVÁ, D. TMEJOVÁ, K. ČÍHALOVÁ, K. KŘÍŽKOVÁ, S. GURÁŇ, R. KOMÍNKOVÁ, M. ŽŮREK, M. KREMPLOVÁ, M. JIMENEZ JIMENEZ, A. NOVOTNÁ, M. HYNEK, D. PEKAŘÍK, V. KIZEK, R.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer).

Klíčová slova

Escherichia coli, Metallothionein, Voltammetry, Cloning, Metal Ionsa

Autoři

ADAM, V.; CHUDOBOVÁ, D.; TMEJOVÁ, K.; ČÍHALOVÁ, K.; KŘÍŽKOVÁ, S.; GURÁŇ, R.; KOMÍNKOVÁ, M.; ŽŮREK, M.; KREMPLOVÁ, M.; JIMENEZ JIMENEZ, A.; NOVOTNÁ, M.; HYNEK, D.; PEKAŘÍK, V.; KIZEK, R.

Rok RIV

2014

Vydáno

10. 9. 2014

ISSN

0013-4686

Periodikum

Electrochimica Acta

Ročník

140

Číslo

Stát

Spojené království Velké Británie a Severního Irska

Strany od

Strany do

Strany počet

BibTex

@article{BUT111094,
  author="Vojtěch {Adam} and Dagmar {Hegerová} and Kateřina {Tmejová} and Kristýna {Číhalová} and Soňa {Křížková} and Roman {Guráň} and Markéta {Komínková} and Michal {Žůrek} and Monika {Kremplová} and Ana Maria {Jimenez Jimenez} and Marie {Novotná} and David {Hynek} and Vladimír {Pekařík} and René {Kizek}",
  title="An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry",
  journal="Electrochimica Acta",
  year="2014",
  volume="140",
  number="SI",
  pages="11--19",
  doi="10.1016/j.electacta.2014.06.091",
  issn="0013-4686"
}