Detail publikace

Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment

NEJDL, L. KUDR, J. ČÍHALOVÁ, K. CHUDOBOVÁ, D. ŽŮREK, M. ŽALUD, L. KOPEČNÝ, L. BURIAN, F. RUTTKAY-NEDECKÝ, B. KŘÍŽKOVÁ, S. NOVOTNÁ, M. HYNEK, D. KOPEL, P. PRÁŠEK, J. ADAM, V. KIZEK, R.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 L. The flow rate was optimized to 500 L/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticlesmodified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per L.

Klíčová slova

Alkaline phosphatase, Bacteria, Electrochemical detection, Magnetic particles, 1-Naphthyl phosphate, Planetary science, Remote sensing

Autoři

NEJDL, L.; KUDR, J.; ČÍHALOVÁ, K.; CHUDOBOVÁ, D.; ŽŮREK, M.; ŽALUD, L.; KOPEČNÝ, L.; BURIAN, F.; RUTTKAY-NEDECKÝ, B.; KŘÍŽKOVÁ, S.; NOVOTNÁ, M.; HYNEK, D.; KOPEL, P.; PRÁŠEK, J.; ADAM, V.; KIZEK, R.

Rok RIV

2014

Vydáno

1. 8. 2014

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

Číslo

Stát

Spolková republika Německo

Strany od

2333

Strany do

2345

Strany počet

BibTex

@article{BUT109831,
  author="Lukáš {Nejdl} and Jiří {Kudr} and Kristýna {Číhalová} and Dagmar {Hegerová} and Michal {Žůrek} and Luděk {Žalud} and Lukáš {Kopečný} and František {Burian} and Branislav {Ruttkay-Nedecký} and Soňa {Křížková} and Marie {Novotná} and David {Hynek} and Pavel {Kopel} and Jan {Prášek} and Vojtěch {Adam} and René {Kizek}",
  title="Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment",
  journal="Electrophoresis",
  year="2014",
  volume="35",
  number="14",
  pages="2333--2345",
  doi="10.1002/elps.201300576",
  issn="0173-0835"
}