Detail publikace

Findings and indication in the PAGE- analysis of sweet protein thaumatin.

VESPALCOVÁ, M. HOLIŠOVÁ, R. VRÁNOVÁ, D. TŮMA, Z.

Originální název

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Thaumatin (E 957) is a low-caloric alternative sweetener produced from fruits of the tropical plant Thaumatococcus danielli Benth.. It is the sweetest known substance, is approximately 3000 times sweeter than sucrose and acts as a taste modifier, too. Chemically, thaumatin is a basic protein, reportedly of extremely high pI = 11.7-12.0 [1] and of relative molecular mass 22 kDa . Commercially available thaumatin preparations consists of two main fractions, thaumatin I and thaumatin II, that differ only by five aminoacids in their chains, and several minor proteinic admixtures. Complete separation of constituents in commercially available thaumatins is very difficult. Well documented analytical technique for the purpose is missing yet. We decided therefore to start systematic research on the thaumatin analysis by exploration on analytical potential of PAGE techniques whose utilization for the purpose is the most frequently reported. Experiments at both non-denaturating and denaturating conditions show that neither thaumatin pI derived from flat bed gel analyses nor efficacy of the SDS-PAGE method for thaumatin, respectively, are reliable. It casts doubt on the capability of flat bed gel analyses to give credible analytical results for thaumatin as the analyte.

Klíčová slova

Thaumatin, PAGE-analysis, SDS-PAGE, basic protein,

Autoři

VESPALCOVÁ, M.; HOLIŠOVÁ, R.; VRÁNOVÁ, D.; TŮMA, Z.

Rok RIV

2005

Vydáno

25. 8. 2005

Nakladatel

Česká společnost chemická

Místo

Brno

ISSN

0009-2770

Periodikum

Chemické listy

Ročník

Číslo

Stát

Česká republika

Strany od

362

Strany do

364

Strany počet

BibTex

@article{BUT42745,
  author="Milena {Vespalcová} and Radana {Holišová} and Dana {Vránová} and Zdeněk {Tůma}",
  title="Findings and indication in the PAGE- analysis of sweet protein thaumatin.",
  journal="Chemické listy",
  year="2005",
  volume="99",
  number="S",
  pages="362--364",
  issn="0009-2770"
}