Detail publikace

An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer

IEREMENKO, A. LIPOVSKÁ, K. KOUŘILOVÁ, X. OBRUČA, S. DVOŘÁK, P.

Originální název

An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The limited number of well-characterised model bacteria cannot address all the challenges in a circular bioeconomy. Therefore, there is a growing demand for new production strains with enhanced resistance to extreme conditions, versatile metabolic capabilities and the ability to utilise cost-effective renewable resources while efficiently generating attractive biobased products. Particular thermophilic microorganisms fulfil these requirements. Non-virulent Gram-negative Caldimonas thermodepolymerans DSM15344 is one such attractive thermophile that efficiently converts a spectrum of plant biomass sugars into high quantities of polyhydroxyalkanoates (PHA)-a fully biodegradable substitutes for synthetic plastics. However, to enhance its biotechnological potential, the bacterium needs to be 'domesticated'. In this study, we established effective homologous recombination and transposon-based genome editing systems for C. thermodepolymerans. By optimising the electroporation protocol and refining counterselection methods, we achieved significant improvements in genetic manipulation and constructed the AI01 chassis strain with improved transformation efficiency and a Delta phaC mutant that will be used to study the importance of PHA synthesis in Caldimonas. The advances described herein highlight the need for tailored approaches when working with thermophilic bacteria and provide a springboard for further genetic and metabolic engineering of C. thermodepolymerans, which can be considered the first model of thermophilic PHA producer.

Klíčová slova

Caldimonas thermodepolymerans; gene deletion; genetic engineering; polyhydroxyalkanoates; thermophiles

Autoři

IEREMENKO, A.; LIPOVSKÁ, K.; KOUŘILOVÁ, X.; OBRUČA, S.; DVOŘÁK, P.

Vydáno

26. 2. 2025

Nakladatel

WILEY

Místo

HOBOKEN

ISSN

1751-7915

Periodikum

Microbial Biotechnology

Ročník

18

Číslo

2

Stát

Spojené státy americké

Strany od

1

Strany do

16

Strany počet

16

URL

https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.70103

BibTex

@article{BUT197285,
  author="Anastasiia {Ieremenko} and Kristýna {Lipovská} and Xenie {Kouřilová} and Stanislav {Obruča} and Pavel {Dvořák}",
  title="An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer",
  journal="Microbial Biotechnology",
  year="2025",
  volume="18",
  number="2",
  pages="1--16",
  doi="10.1111/1751-7915.70103",
  issn="1751-7915",
  url="https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.70103"
}