Detail publikace

Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

JUREČKOVÁ, K. NYKRÝNOVÁ, M. SLANINOVÁ, E. FLEURIOT-BLITMAN, H. AMSTUTZ, V. HEŘMÁNKOVÁ, K. BEZDÍČEK, M. MRÁZOVÁ, K. HRUBANOVÁ, K. ZINN, M. OBRUČA, S. SEDLÁŘ, K.

Originální název

Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs.

Klíčová slova

Rhodospirillum rubrum; RNA-Seq; Transcriptome; Genome; Depolymerase knock-out; Gene ontology; Metabolism; Fructose; Acetate; Polyhydroxyalkanoates

Autoři

JUREČKOVÁ, K.; NYKRÝNOVÁ, M.; SLANINOVÁ, E.; FLEURIOT-BLITMAN, H.; AMSTUTZ, V.; HEŘMÁNKOVÁ, K.; BEZDÍČEK, M.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; ZINN, M.; OBRUČA, S.; SEDLÁŘ, K.

Vydáno

26. 6. 2024

Nakladatel

Elsevier

ISSN

2001-0370

Periodikum

Computational and Structural Biotechnology Journal

Ročník

23

Číslo

December 2024

Stát

Švédské království

Strany od

2681

Strany do

2694

Strany počet

14

URL

https://www.sciencedirect.com/science/article/pii/S2001037024002204

Plný text v Digitální knihovně

http://hdl.handle.net/11012/249379

BibTex

@article{BUT188890,
  author="Kateřina {Šabatová} and Markéta {Jakubíčková} and Eva {Slaninová} and Hugo {Fleuriot-Blitman} and Véronique {Amstutz} and Kristýna {Heřmánková} and Matěj {Bezdíček} and Kateřina {Mrázová} and Kamila {Hrubanová} and Manfred {Zinn} and Stanislav {Obruča} and Karel {Sedlář}",
  title="Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum",
  journal="Computational and Structural Biotechnology Journal",
  year="2024",
  volume="23",
  number="December 2024",
  pages="2681--2694",
  doi="10.1016/j.csbj.2024.06.023",
  issn="2001-0370",
  url="https://www.sciencedirect.com/science/article/pii/S2001037024002204"
}