Detail publikace

Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems

MIŠÍK, O. SZABOVÁ, J. CEJPEK, O. MALÝ, M. JUGL, A. BĚLKA, M. MRAVEC, F. LÍZAL, F.

Originální název

Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Liposomal carrier systems have emerged as a promising technology for pulmonary drug delivery. This study focuses on two selected liposomal systems, namely, dipalmitoylphosphatidylcholine stabilized by phosphatidic acid and cholesterol (DPPC-PA-Chol) and dipalmitoylphosphatidylcholine stabilized by polyethylene glycol and cholesterol (DPPC-PEG-Chol). First, the research investigates the stability of these liposomal systems during the atomization process using different kinds of nebulizers (air-jet, vibrating mesh, and ultrasonic). The study further explores the aerodynamic particle size distribution of the aerosol generated by the nebulizers. The nebulizer that demonstrated optimal stability and particle size was selected for more detailed investigation, including Andersen cascade impactor measurements, an assessment of the influence of flow rate and breathing profiles on aerosol particle size, and an in vitro deposition study on a realistic replica of the upper airways. The most suitable combination of a nebulizer and liposomal system was DPPC-PA-Chol nebulized by a Pari LC Sprint Star in terms of stability and particle size. The influence of the inspiration flow rate on the particle size was not very strong but was not negligible either (decrease of D-v50 by 1.34 mu m with the flow rate increase from 8 to 60 L/min). A similar effect was observed for realistic transient inhalation. According to the in vitro deposition measurement, approximately 90% and 70% of the aerosol penetrated downstream of the trachea using the stationary flow rate and the realistic breathing profile, respectively. These data provide an image of the potential applicability of liposomal carrier systems for nebulizer therapy. Regional lung drug deposition is patient-specific; therefore, deposition results might vary for different airway geometries. However, deposition measurement with realistic boundary conditions (airway geometry, breathing profile) brings a more realistic image of the drug delivery by the selected technology. Our results show how much data from cascade impactor testing or estimates from the fine fraction concept differ from those of a more realistic case.

Klíčová slova

liposome; aerosol; particle size; nebulizer; pulmonary drug delivery; inhalation; deposition

Autoři

MIŠÍK, O.; SZABOVÁ, J.; CEJPEK, O.; MALÝ, M.; JUGL, A.; BĚLKA, M.; MRAVEC, F.; LÍZAL, F.

Vydáno

11. 3. 2024

Nakladatel

American Chemical Society

Místo

WASHINGTON

ISSN

1543-8384

Periodikum

MOLECULAR PHARMACEUTICS

Ročník

21

Číslo

4

Stát

Spojené státy americké

Strany od

1848

Strany do

1860

Strany počet

13

URL

https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.3c01146

Plný text v Digitální knihovně

http://hdl.handle.net/11012/245516

BibTex

@article{BUT188404,
  author="Ondrej {Mišík} and Jana {Szabová} and Ondřej {Cejpek} and Milan {Malý} and Adam {Jugl} and Miloslav {Bělka} and Filip {Mravec} and František {Lízal}",
  title="Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems",
  journal="MOLECULAR PHARMACEUTICS",
  year="2024",
  volume="21",
  number="4",
  pages="13",
  doi="10.1021/acs.molpharmaceut.3c01146",
  issn="1543-8384",
  url="https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.3c01146"
}