Detail publikace

Variability of Inverted Repeats in All Available Genomes of Bacteria

PORUBIAKOVÁ, O. HAVLÍK, J. INDU, M. ŠEDŸ, M. PŘEPECHALOVÁ, V. BARTAS, M. BIDULA, S. ŠŤASTNÝ, J. FOJTA, M. BRÁZDA, V.

Originální název

Variability of Inverted Repeats in All Available Genomes of Bacteria

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Noncanonical secondary structures in nucleic acids have been studied intensively in recent years. Important biological roles of cruciform structures formed by inverted repeats (IRs) have been demonstrated in diverse organisms, including humans. Using Palindrome analyser, we analyzed IRs in all accessible bacterial genome sequences to determine their frequencies, lengths, and localizations. IR sequences were identified in all species, but their frequencies differed significantly across various evolutionary groups. We detected 242,373,717 IRs in all 1,565 bacterial genomes. The highest mean IR frequency was detected in the Tenericutes (61.89 IRs/kbp) and the lowest mean frequency was found in the Alphaproteobacteria (27.08 IRs/kbp). IRs were abundant near genes and around regulatory, tRNA, transfer-messenger RNA (tmRNA), and rRNA regions, pointing to the importance of IRs in such basic cellular processes as genome maintenance, DNA replication, and transcription. Moreover, we found that organisms with high IR frequencies were more likely to be endosymbiotic, antibiotic producing, or pathogenic. On the other hand, those with low IR frequencies were far more likely to be thermophilic. This first comprehensive analysis of IRs in all available bacterial genomes demonstrates their genomic ubiquity, nonrandom distribution, and enrichment in genomic regulatory regions.IMPORTANCE Our manuscript reports for the first time a complete analysis of inverted repeats in all fully sequenced bacterial genomes. Thanks to the availability of unique computational resources, we were able to statistically evaluate the presence and localization of these important regulatory sequences in bacterial genomes. This work revealed a strong abundance of these sequences in regulatory regions and provides researchers with a valuable tool for their manipulation. Our manuscript reports for the first time a complete analysis of inverted repeats in all fully sequenced bacterial genomes. Thanks to the availability of unique computational resources, we were able to statistically evaluate the presence and localization of these important regulatory sequences in bacterial genomes. This work revealed a strong abundance of these sequences in regulatory regions and provides researchers with a valuable tool for their manipulation.

Klíčová slova

inverted repeats; Palindrome analyser; bacteria domain; bacterial genome analysis

Autoři

PORUBIAKOVÁ, O.; HAVLÍK, J.; INDU, M.; ŠEDŸ, M.; PŘEPECHALOVÁ, V.; BARTAS, M.; BIDULA, S.; ŠŤASTNÝ, J.; FOJTA, M.; BRÁZDA, V.

Vydáno

26. 6. 2023

Nakladatel

American Society for Microbiology

Místo

WASHINGTON

ISSN

2165-0497

Periodikum

Microbiology spectrum

Ročník

11

Číslo

4

Stát

Spojené státy americké

Strany od

1

Strany do

11

Strany počet

11

URL

https://journals.asm.org/doi/10.1128/spectrum.01648-23

Plný text v Digitální knihovně

http://hdl.handle.net/11012/244957

BibTex

@article{BUT184578,
  author="Otília {Porubiaková} and Jan {Havlík} and Michal {Indu} and Michal {Šedý} and Veronika {Přepechalová} and Martin {Bartas} and Stefan {Bidula} and Jiří {Šťastný} and Miroslav {Fojta} and Václav {Brázda}",
  title="Variability of Inverted Repeats in All Available Genomes of Bacteria",
  journal="Microbiology spectrum",
  year="2023",
  volume="11",
  number="4",
  pages="11",
  doi="10.1128/spectrum.01648-23",
  issn="2165-0497",
  url="https://journals.asm.org/doi/10.1128/spectrum.01648-23"
}