Detail publikace

Isolation and identification of genus Fragaria DNA in cosmetic products

ROMANOVSKÁ, D. FIALOVÁ, L. MÁROVÁ, I.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Cosmetic products with ingredients of plant origin promises beneficial effects on the customer health. It is possible to detect presence of plant DNA in the cosmetic product in very small quantities using techniques of molecular biotechnology and, thus, to detect the presence of a potential allergen that may negativelly affects the health of sensitive individuals. One of these allergens is cinnamyl alcohol [1] presented for example in fruits that belong to genus Fragaria. In this study gene-specific primers encoding cinnamyl alcohol dehydrogenase were used for detection of presence of Fragaria x ananassa DNA in leave-on cosmetic product [2]. Purified DNA was isolated and the specific sequence was amplified using real-time PCR [3]. The presence of amplicons of specific size and melt temperature was confirmed using agarose electrophoresis and HRM analysis.

Klíčová slova

plant DNA, cinamyl alcohol, Fragaria, real-time PCR

Autoři

ROMANOVSKÁ, D.; FIALOVÁ, L.; MÁROVÁ, I.

Vydáno

24. 9. 2019

Strany počet

BibTex

@misc{BUT162322,
  author="Denisa {Langová} and Lenka {Fialová} and Ivana {Márová}",
  title="Isolation and identification of genus Fragaria DNA in cosmetic products",
  year="2019",
  pages="1",
  note="abstract"
}