Detail publikace

Interconnection of Waste Chicken Feather Biodegradation and Keratinase and mcl-PHA Production Employing Pseudomonas putida KT2440

PERNICOVÁ, I. ENEV, V. MÁROVÁ, I. OBRUČA, S.

Originální název

Interconnection of Waste Chicken Feather Biodegradation and Keratinase and mcl-PHA Production Employing Pseudomonas putida KT2440

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background and objective: Waste chicken feather is an important waste product of the poultry processing industry and is annually produced in substantial amounts. Hence, wise management of this waste is desirable. In this work we aimed at feathers biodegradation by a selected bacterial strain capable of utilizing chicken feathers as sole carbon source-Pseudomonas putida KT2440. To utilize feather, the bacterial culture excrete keratinase, which can be easily isolated after biodegradation process and which, therefore, represents an interesting side product of the intended technology. Moreover, bacterial culture of employed for feather degradation is also capable of mcl-PHA accumulation. Materials and methods: Bacterial culture of Pseudomonas putida KT2440 was cultivated in presence of waste chicken feathers as the only carbon source; during the cultivation keratinase activity and biomass growth were monitored. Metabolically active biomass after feather degradation was used for mcl-PHA production. Results and conclusion: During cultivation on waste feathers, bacteria did not accumulate detectable amounts of medium-chain length polyhydroxyalkanoate (mcl-PHA); nevertheless, when metabolically active bacterial cells after feather biodegradation were transferred into nitrogen limited mineral media, a high medium-chain length polyhydroxyalkanoate content of 61% of cell dry weight in microbial cells was reached. The polymer consisted of 3-hydroxyhexanoate (27.2% mol) and 3-hydroxyoctanoate (72.8% mol) monomer units. Therefore, this work demonstrates a possible interconnection of feather biodegradation with keratinase and medium-chain length polyhydroxyalkanoate production.

Klíčová slova

Waste feathers; Pseudomonas putida; keratinase; mcl-PHA

Autoři

PERNICOVÁ, I.; ENEV, V.; MÁROVÁ, I.; OBRUČA, S.

Vydáno

1. 6. 2019

Nakladatel

SHAHID BEHESHTI UNIV MEDICAL SCIENCES, FAC MED

Místo

TEHRAN

ISSN

2345-5357

Periodikum

APPLIED FOOD BIOTECHNOLOGY

Ročník

6

Číslo

2345-5357

Stát

Íránská islámská republika

Strany od

83

Strany do

90

Strany počet

8

URL

https://www.google.cz/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwj1uN_80N3nAhVTh1wKHTTkDFYQFjACegQIAhAB&url=http%3A%2F%2Fjournals.sbmu.ac.ir%2Fafb%2Farticle%2Fview%2F21429&usg=AOvVaw3eqA2hZUKMTPtos_x6IO_H

BibTex

@article{BUT162108,
  author="Iva {Buchtíková} and Vojtěch {Enev} and Ivana {Márová} and Stanislav {Obruča}",
  title="Interconnection of Waste Chicken Feather Biodegradation and Keratinase and mcl-PHA Production Employing Pseudomonas putida KT2440",
  journal="APPLIED FOOD BIOTECHNOLOGY",
  year="2019",
  volume="6",
  number="2345-5357",
  pages="83--90",
  doi="10.22037/afb.v6i1.21429",
  issn="2345-5357",
  url="https://www.google.cz/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwj1uN_80N3nAhVTh1wKHTTkDFYQFjACegQIAhAB&url=http%3A%2F%2Fjournals.sbmu.ac.ir%2Fafb%2Farticle%2Fview%2F21429&usg=AOvVaw3eqA2hZUKMTPtos_x6IO_H"
}