Implementation of industrial approach of X-ray computed microtomography in developmental biology

abstrakt

angličtina

X-ray computed microtomography (microCT) is a non-destructive method for imaging inner structure of materials. Thanks to the possibility of fast measurement (in order of minutes to hours) and simple preparation of the sample, this method finds application not only in industry or medicine, but also in scientific fields including developmental biology. Modern developmental biology requires both qualitative and quantitative 3D information about studied objects, which is not usually provided by conventional 2D imaging methods. In addition, the complexity of biological structures often requires a comprehensive approach for quantitative comparison including determination of dimension, surface or volume. The goal of this work is to provide connection between technical and biological field. It brings new possibilities in imaging, data processing and evaluation of 3D models. Diverse structures were analyzed by different approaches on the selected examples from developmental biology. In addition, quantitative analysis usually used in industry were applied to segmented 3D models e.g. wall thickness analysis of facial cartilage, shape comparisons of nasal capsules, automatic quantification of the cells in the limb etc. A complete procedure consisting of staining, microCT measurement and subsequent data processing is discussed.

CT, tomography, 3D, imaging. biology

TESAŘOVÁ, M.; KAUCKÁ, M.; ADAMEYKO, I.; NOVOTNÁ, M.; ZIKMUND, T.; KAISER, J.

5. 7. 2018

AMCA spol s.r.o.

Prague, Czech Republic

978-80-88214-11-3

XXVI International Symposium on Morphological Sciences 2018 - Book of Abstracts

1st edition

73

73

90