Polylysine Coated Magnetic Nanoparticles As A Tool For DNA Isolation From Real Samples

abstrakt

angličtina

Solid phase DNA extraction has been shown to be a simple method for PCR-ready DNA isolation. Poly(Llysine) coated iron oxide nanoparticles (MNPs) were not studied so far. Poly(L-lysine) coated solid surfaces are positively charged in acidic pH and negatively charged in alkali pH due to the protonation and deprotonation of functional groups. This enables the binding of charged species, including nucleic acids, using electrostatic interaction between the solid surface and the charged molecules. First, Nicotiana tabacum DNA capture and elution was studied with different amounts of poly(Llysine) coated magnetic nanoparticles. Buffers with different acidic pH (4.0 – 6.5) and alcalic pH (7.5 - 10.0) were tested for DNA capture or elution, respectively (Figure 1). Next, MNPs were used for isolation of PCRready DNA from plant based foods, in particular from chokeberry, red currant and plum jams which are rich in PCR inhibitors (phenolic compounds and polysacharides) and contain DNA degraded during technological processing. Differently purified food homogenates were prepared for DNA isolation. It was found out that the isolated DNAs contained different amounts of impurities according to the purification steps used for preparation of homogenates from different food products. Some DNAs were successfully amplified in conventional PCR.

magnetic nanoparticles, poly(L-lysine), DNA isolation, real samples

FIALOVÁ, L.; ŠPANOVÁ, A.; RITTICH, B.

12. 9. 2018

Brno University of Technology, Faculty of Chemistry, Purkyňova 464/118 612 00 Brno

Brno

978-80-214-5488-0

7th Meeting on Chemistry and Life 2018. Book of abstracts

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