Detail publikace

DNA based identification of microorganisms in a probiotic products

ČAKAJDOVÁ, M. TRACHTOVÁ, Š. ŠPANOVÁ, A. HORÁK, D. RITTICH, B.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Brewers yeasts (Saccharomyces cerevisiae) are a secondary product from production of beer. They are used as a nutritional supplement for long time, too. Brewers yeasts can be supplemented with probiotic bacteria and roughage and with vitamins, minerals and enzymes, too. Brewers yeast tablets with content of genus Lactobacillus and Biffidobacterium were used for analysis. Two types of samples were before expiration, and other were after expiration (1 and 8 years). DNA was isolated from crude cell lysates of brewers yeast tablets using magnetics P(HEMA-co-GMA) microspheres covered by carboxyl groups. Non-diluted DNA was used for analysis of Lactobacillus and Biffidobacterium. The conventional polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) were used for amplification of DNAs.

Klíčová slova

probiotic products, polymerase chain reaction, real time polymerase chain reaction, DNA isolation

Autoři

ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.

Vydáno

28. 5. 2013

Nakladatel

Institute of Immunology and Experimental Therapy Polish academy of Science

Místo

Kudowa Zdrój, Polsko

ISBN

978-83-928488-3-7

Kniha

Microbiology and Immunology of Mucosa Probiotics Conference 2013

Edice

Číslo edice

Strany od

Strany do

Strany počet

BibTex

@misc{BUT99950,
  author="Martina {Čakajdová} and Štěpánka {Trachtová} and Alena {Španová} and Daniel {Horák} and Bohuslav {Rittich}",
  title="DNA based identification of microorganisms in a probiotic products",
  booktitle="Microbiology and Immunology of Mucosa Probiotics Conference 2013",
  year="2013",
  series="1",
  edition="1",
  pages="41--41",
  publisher="Institute of Immunology and Experimental Therapy Polish academy of Science",
  address="Kudowa Zdrój, Polsko",
  isbn="978-83-928488-3-7",
  note="abstract"
}