Detail publikace

Development of Simple Splitless Gradient Liquid Chromatographic system

ŠESTÁK, J. a kol.

Originální název

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

A simple gradient liquid chromatographic system for micro and nano column separations has been assembled and tested. The device involves simple splitless gradient generation and sample delivery. Experimental configuration consists of an OEM programmable syringe pump equipped with a glass microsyringe and ten-port selector valve. Automated system was controlled via LabVIEW development environment. Continuous gradients of mobile phase were created in the syringe barrel by successive sucking of adequate volumes of four mobile phases with gradually decreasing acetonitrile concentration and due to turbulent mixing occurred at the syringe needle-barrel boundary. System performance was evaluated using test mixture of six alkylphenones. Good values of relative standard deviation of retention times were received (intra-day RSD < 0.3 %, inter-day RSD < 1.4 %). Repeatability of sample injection in the range 0.1 – 3 ul was evaluated. The peak areas were measured and statistically evaluated (RSD < 5 %).

Klíčová slova

splitless gradient, nano column, alkylphenones

Autoři

ŠESTÁK, J. a kol.

Rok RIV

2012

Vydáno

7. 12. 2012

Nakladatel

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

ISBN

978-80-214-4644-1

Kniha

Studentská odborná konference Chemie je život - Sborník příspěvků

Strany od

446

Strany do

449

Strany počet

BibTex

@inproceedings{BUT97457,
  author="Jozef {Šesták}",
  title="Development of Simple Splitless Gradient Liquid Chromatographic system",
  booktitle="Studentská odborná konference Chemie je život - Sborník příspěvků",
  year="2012",
  pages="446--449",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  isbn="978-80-214-4644-1"
}