Detail publikace

Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.

PROSECKÁ, E. RAMPICHOVÁ, M. VOJTOVÁ, L. TVRDÍK, D. MELČÁKOVÁ, Š. JUHASOVÁ, J. PLCNER, M. JAKUBOVÁ, R. JANČÁŘ, J. NEČAS, A. KOCHOVÁ, P. KLEPÁČEK, J. TONAR, Z. AMLER, E.

Originální název

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution.

Klíčová slova

collagen/HA scaffold;MSCs;osteoblasts;pore size;mechanical testing

Autoři

PROSECKÁ, E.; RAMPICHOVÁ, M.; VOJTOVÁ, L.; TVRDÍK, D.; MELČÁKOVÁ, Š.; JUHASOVÁ, J.; PLCNER, M.; JAKUBOVÁ, R.; JANČÁŘ, J.; NEČAS, A.; KOCHOVÁ, P.; KLEPÁČEK, J.; TONAR, Z.; AMLER, E.

Rok RIV

2011

Vydáno

19. 8. 2011

Nakladatel

WILEY PERIODICALS

Místo

USA

ISSN

1549-3296

Periodikum

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Ročník

99A

Číslo

Stát

Spojené státy americké

Strany od

307

Strany do

315

Strany počet

BibTex

@article{BUT89272,
  author="PROSECKÁ, E. and RAMPICHOVÁ, M. and VOJTOVÁ, L. and TVRDÍK, D. and MELČÁKOVÁ, Š. and JUHASOVÁ, J. and PLCNER, M. and JAKUBOVÁ, R. and JANČÁŘ, J. and NEČAS, A. and KOCHOVÁ, P. and KLEPÁČEK, J. and TONAR, Z. and AMLER, E.",
  title="Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.",
  journal="JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A",
  year="2011",
  volume="99A",
  number="2",
  pages="307--315",
  issn="1549-3296"
}