Detail publikace

Finishing the S.pombe genome deletion project. An update.

ČARNECKÁ, M. MÁROVÁ, I.

Originální název

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.

Klíčová slova

fission yeast; gene deletion; genomics; essential genes; vectors

Autoři

ČARNECKÁ, M.; MÁROVÁ, I.

Rok RIV

2010

Vydáno

28. 7. 2010

Nakladatel

LANDES BIOSCIENCE

Místo

USA

ISSN

1538-4101

Periodikum

Cell Cycle

Ročník

Číslo

Stát

Spojené státy americké

Strany od

2399

Strany do

2402

Strany počet

BibTex

@article{BUT50225,
  author="Martina {Čarnecká} and Ivana {Márová}",
  title="Finishing the S.pombe genome deletion project. An update.",
  journal="Cell Cycle",
  year="2010",
  volume="9",
  number="12",
  pages="2399--2402",
  issn="1538-4101"
}