Detail publikace

Dynamics of hyaluronan aqueous solutions as assessed by fast field cycling NMR relaxometry

PRŮŠOVÁ, A. CONTE, P. KUČERÍK, J.ALONZO, G.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Fast field cycling (FFC) NMR relaxometry has been used to study the conformational properties of aqueous solutions of hyaluronan (HYA) at three concentrations in the range 10 to 25 mg mL(-1). Results revealed that, irrespective of the solution concentration, three different hydration layers surround hyaluronan. The inner layer consists of water molecules strongly retained in the proximity of the HYA surface. Because of their strong interactions with HYA, water molecules in this inner hydration layer are subject to very slow dynamics and have the largest correlation times. The other two hydration layers are made of water molecules which are located progressively further from the HYA surface. As a result, decreasing correlation times caused by faster molecular motion were measured. The NMRD profiles obtained by FFC-NMR relaxometry also showed peaks attributable to H-1-N-14 quadrupole interactions. Changes in intensity and position of the quadrupolar peaks in the NMRD profiles suggested that with increasing concentration the amido group is progressively involved in the formation of weak and transient intramolecular water bridging adjacent hyaluronan chains. In this work, FFC-NMR was used for the first time to obtain deeper insight into HYA-water interactions and proved itself a powerful and promising tool in hyaluronan chemistry.

Klíčová slova

FFC-NMR; Relaxometry; Correlation time; Quadrupole interactions; Hydration layer; Hyaluronan

Autoři

PRŮŠOVÁ, A.; CONTE, P.; KUČERÍK, J.;ALONZO, G.

Rok RIV

2010

Vydáno

20. 8. 2010

Nakladatel

Springer

Místo

HEIDELBERG, GERMANY

ISSN

1618-2642

Periodikum

Analytical and Bioanalytical Chemistry

Ročník

397

Číslo

Stát

Spolková republika Německo

Strany od

3023

Strany do

3028

Strany počet

BibTex

@article{BUT49837,
  author="PRŮŠOVÁ, A. and CONTE, P. and KUČERÍK, J. and ALONZO, G.",
  title="Dynamics of hyaluronan aqueous solutions as assessed by fast field cycling NMR relaxometry",
  journal="Analytical and Bioanalytical Chemistry",
  year="2010",
  volume="397",
  number="7",
  pages="3023--3028",
  issn="1618-2642"
}