Impact of plasma treated liquids on pathogenic bacteria

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angličtina

In this work, plasma treated liquids (PTLs) were prepared by four different plasma systems: the pinhole discharge with DC or AC high voltage source (PTLAC and PTLDC), the liquid electrode dielectric barrier discharge (PTLDBD), and the microwave surface wave discharge (PTLSW). Prepared PTLs were first characterized in terms of reactive particles (hydrogen peroxide, nitrates and nitrites) and changes in specific conductivity and pH. This was followed by microbiological testing of the antimicrobial efficiency of the prepared PTL. The bacteria Staphylococcus pseudintermedius and Pseudomonas aeruginosa were selected as testing microorganisms. These microorganisms were exposed to PTL, and the effects were studied for exposure times from 1 minute to 4 hours. After 24 hours of cultivation, the antimicrobial effects were evaluated by the standard plate count method and also by the image analysis in the software Aurora. The next step was to compile antibiograms and compare the effect of the prepared PTL in different systems with the effect of antibiotics. It was proven that the bacterium Pseudomonas aeruginosa is resistant to several antibiotics. Thus, the prepared PTLDC could represent the possibility of its inactivation without the use of antibiotics. Staphylococcus pseudintermedius was not resistant to any of the tested antibiotics, but with an incorrect use there is a risk of developing resistance. Which is why PTLs, with the highest antimicrobial effect, were also found for this microorganism.

antimicrobial effects, Candida glabrata, CAP, cold plasma, indirect treatment

TREBULOVÁ, K.; KOREČKOVÁ, S.; PALIČKOVÁ, I; KOZÁKOVÁ, Z.; HRUDKA, J.; ČÍŽEK, A.; KRČMA, F.

15. 4. 2024

University of Orleans

Orleans

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