Detail publikace

Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles

MURA, M. CARUCCI, C. CADDEO, E. SOVOVÁ, Š. PILUDU, M. PEKAŘ, M. JACHIMSKA, B. PARSONS, D. E. SALIS, A.

Originální název

Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The effect of buffer species on biomolecules and biomolecule-nanoparticle interactions is a phenomenon that has been either neglected, or not understood. Here, we study the formation of a BSA protein corona (PC) around amino-functionalized mesoporous silica nanoparticles (MSN-NH2) 2 ) in the presence of different buffers (Tris, BES, cacodylate, phosphate, and citrate) at the same pH (7.15) and different concentrations (10, 50, and 100 mM). We find that BSA adsorption is buffer specific, with the adsorbed amount of BSA being 4.4 times higher in the presence of 100 mM Tris (184 +/- 3 mg/g) than for 100 mM citrate (42 +/- 2 mg/g). That is a considerable difference that cannot be explained by conventional theories. The results become clearer if the interaction energies between BSA and MSN-NH2, 2 , considering the electric double layer (EEDL) EDL ) and the van der Waals (EvdW) vdW ) terms, are evaluated. The buffer specific PC derives from buffer specific zeta potentials that, for MSN-NH2, are positive with Tris and negative with citrate buffers. A reversed sign of zeta potentials can be obtained by considering polarizability-dependent dispersion forces acting together with electrostatics to give the buffer specific outcome. These results are relevant not only to our understanding of the formation of the PC but may also apply to other bio- and nanosystems in biological media.

Klíčová slova

buffer; protein corona; silica nanoparticles

Autoři

MURA, M.; CARUCCI, C.; CADDEO, E.; SOVOVÁ, Š.; PILUDU, M.; PEKAŘ, M.; JACHIMSKA, B.; PARSONS, D. E.; SALIS, A.

Vydáno

5. 8. 2024

Nakladatel

ACADEMIC PRESS INC ELSEVIER SCIENCE

Místo

SAN DIEGO

ISSN

0021-9797

Periodikum

Journal of Colloid and Interface Science

Ročník

677

Číslo

January

Stát

Spojené státy americké

Strany od

540

Strany do

547

Strany počet

8

URL

https://www.sciencedirect.com/science/article/pii/S0021979724017727

BibTex

@article{BUT189337,
  author="MURA, M. and CARUCCI, C. and CADDEO, E. and SOVOVÁ, Š. and PILUDU, M. and PEKAŘ, M. and JACHIMSKA, B. and PARSONS, D. E. and SALIS, A.",
  title="Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles",
  journal="Journal of Colloid and Interface Science",
  year="2024",
  volume="677",
  number="January",
  pages="540--547",
  doi="10.1016/j.jcis.2024.07.258",
  issn="0021-9797",
  url="https://www.sciencedirect.com/science/article/pii/S0021979724017727"
}