Detail publikace

High-resolution LA-ICP-MS and tracing Ru-based metallocene in tumour cells

Kristýna Bilavčíková, Michaela Vašinová Galiová, Marie Novotná, Roman Hrstka, Jiří Pinkas, Jindřich Karban

Originální název

Typ

konferenční sborník (ne článek)

Jazyk

angličtina

Originální abstrakt

In this work LA-ICP-MS were employed for tracing a novel unique Ru-based drug in lung cancer cell lines. The implementation of Aerosol Rapid Introduction System and experimental parameters fit-for-purpose of high spatial resolution 2D single cell imaging at ultratrace level enabled to localize Ru in the cell nuclei. Furthermore, the laser ablation sampling was employed to clarify kinetics mechanism, when Ru-based drug cell influx was determined 6, 12 and 24 hours after drug application. These results provide essential information about drug uptake by cells, and at the same time, together with other advanced analytical methods, they can complement the set of information needed to obtain a comprehensive result in the sense of determining the mechanism of cell line resistance.

Klíčová slova

LA-ICP-MS; high-resolution; multi-elemental analysis; 2D imaging; single cell analysis

Autoři

Kristýna Bilavčíková, Michaela Vašinová Galiová, Marie Novotná, Roman Hrstka, Jiří Pinkas, Jindřich Karban

Vydáno

2. 7. 2024

ISBN

978-94-6498-854-3

Kniha

Book of abstracts of the 16th European Workshop on Laser Ablation (EWLA2024)

BibTex

@proceedings{BUT189076,
  editor="Kristýna {Bilavčíková} and Michaela {Vašinová Galiová} and Marie {Novotná}",
  title="High-resolution LA-ICP-MS and tracing Ru-based metallocene in tumour cells",
  year="2024",
  isbn="978-94-6498-854-3"
}