Detail publikace
Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes
PAVLIŇÁKOVÁ, V. IZSÁK, D. LIPOVÝ, B. KNOZ, M. SMOLNICKÁ, A. FALDYNA, M. JEKLOVÁ, E. KOUTNÁ, I. VOJTOVÁ, L.
Originální název
Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes
Anglický název
Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes
Typ
abstrakt
Jazyk
čeština
Originální abstrakt
The skin, the body‘s largest organ, serves as a vital barrier protecting against external elements and pathogens. Comprising the epidermis, dermis, and hypodermis, it plays a crucial role in protecting the body. This study focuses on developing a multilayered 4th generation scaffold. The epidermal layer was fabricated using elatin/polycaprolactone/oxidized cellulose nanofibers, while the dermis and hypodermis were formed from a 3D porous scaffold made of collagen type I and antimicrobial chitosan, differing only in scaffold thickness. These layers were crosslinked and characterized using various physicochemical methods, including scanning electron microscopy to assess morphology and pore size. Subsequently, the dermis and hypodermis-mimicking scaffolds were seeded with differentiated mesenchymal stem cells into fibroblasts and preadipocytes. Isolated keratinocytes were then applied atop the fibrous layer to mimic the epidermis. The initial successful in vivo testing of these skin substitutes carried out in a pig model represents a significant step towards their potential clinical application.
Anglický abstrakt
The skin, the body‘s largest organ, serves as a vital barrier protecting against external elements and pathogens. Comprising the epidermis, dermis, and hypodermis, it plays a crucial role in protecting the body. This study focuses on developing a multilayered 4th generation scaffold. The epidermal layer was fabricated using elatin/polycaprolactone/oxidized cellulose nanofibers, while the dermis and hypodermis were formed from a 3D porous scaffold made of collagen type I and antimicrobial chitosan, differing only in scaffold thickness. These layers were crosslinked and characterized using various physicochemical methods, including scanning electron microscopy to assess morphology and pore size. Subsequently, the dermis and hypodermis-mimicking scaffolds were seeded with differentiated mesenchymal stem cells into fibroblasts and preadipocytes. Isolated keratinocytes were then applied atop the fibrous layer to mimic the epidermis. The initial successful in vivo testing of these skin substitutes carried out in a pig model represents a significant step towards their potential clinical application.
Klíčová slova
bilayer scaffold, skin, nanofibers, skin substitutes
Klíčová slova v angličtině
bilayer scaffold, skin, nanofibers, skin substitutes
Autoři
PAVLIŇÁKOVÁ, V.; IZSÁK, D.; LIPOVÝ, B.; KNOZ, M.; SMOLNICKÁ, A.; FALDYNA, M.; JEKLOVÁ, E.; KOUTNÁ, I.; VOJTOVÁ, L.
Vydáno
25. 4. 2024
Místo
Praha
ISBN
978-80-01-07293-6
Kniha
Bioimplantologie a regenerativní medicína 2024
Číslo edice
1. elektronické
Strany od
43
Strany do
43
Strany počet
59
URL
BibTex
@misc{BUT188633,
author="Veronika {Pavliňáková} and Dávid {Izsák} and Břetislav {Lipový} and Martin {Knoz} and Anna {Smolnická} and Martin {Faldyna} and Edita {Jeklová} and Irena {Koutná} and Lucy {Vojtová}",
title="Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes",
booktitle="Bioimplantologie a regenerativní medicína 2024",
year="2024",
edition="1. elektronické",
pages="43--43",
address="Praha",
isbn="978-80-01-07293-6",
url="https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000499-73ed673ed8/bioimplantologie_2024_sbornik.pdf?ph=921c88450d",
note="abstract"
}