Inducing of biological and nutritional stress in cultivation of carotenogenic yeast and autotrophic microalgae

abstrakt

angličtina

This study was focused on the effect of biological and nutritional stress induced on various autotrophic microalgae and carotenogenic yeast. Nutritional stress was ensured by adding selected waste oils (coffee and frying oil) to the media, and biological stress was achieved by cultivating microorganisms within one medium in so-called co-cultivations. The monitored properties were the production of biomass and metabolites and production of lipids and lipophilic substances. All types of these stresses tended to lead to an increase in production of both biomass and monitored metabolites. The studied metabolites were carotenoids, chlorophylls, coenzyme Q, sterols and fatty acids. The studied yeast was Rhodotorula kratochvilovae and Rhodotorula toruloides. Representatives of microalgae were Desmodesmus acutus, Scenedesmus obliquus and Desmodesmus communis. The addition of waste oils in co-cultivations with yeast R.kratochvilovae tended to have the best production of both biomass and metabolites compared to other tested yeast. The best co-cultivation experiments with added waste oil showed up to be R. kratochvilovae with Desmodesmus communis and R. kratochvilovae with Scenedesmus obliquus. In microalgae experiments, it was found that nutritional stress in the form of added glycerol to the medium had inhibitory effect on the growth and metabolism of microalgae.

microalgae, yeast, waste substrates, co-cultivations, fatty acids, metabolites

SIKOROVÁ, P.; SZOTKOWSKI, M.; MÁROVÁ, I.

5. 10. 2023