POLY(3-HYDROXYBUTYRATE) BASED MATERIALS FOR 3D PRINTING OF MEDICAL APPLICATIONS FOR TISSUE ENGINEERING

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One of the most advanced approaches used in contemporary regenerative medicine is tissue engineering. The key role in tissue engineering is played by a porous structure (scaffold), which serves as a support for cells and is then integrated into the human body. The scaffold must have a suitable porosity and 3D structure for the cells to grow through the entire volume of it and form new tissue. In recent years, a method of 3D printing is capturing attention in the bone tissue engineering field as it allows complex tailor-made structures to be produced in a time and cost-effective manner for every specific case and patient. Some bioplastics, such as polylactic acid, poly(3-hydroxybutyrate), or polycaprolactone are appealing not only for their environmental friendliness but also for their biocompatibility. Furthermore, tricalcium phosphate as well as hydroxyapatite can be used as a bioactive filler to promote in vivo osteogenic differentiation of mesenchymal stem cells. The research focuses on 3D printing and testing of P3HB-based scaffolds. Three polymer blends were prepared based on either commercial poly(3-hydroxybutyrate) or P3HB from the chloroform-free extraction route, poly(lactic acid) and polycaprolactone, oligomeric adipate ester plasticizer, and tricalcium phosphate as a bioactive filler, and processed into the form of 3D printing filaments. The temperature tower test and warping test were conducted to determine the processing conditions for 3D printing. Mechanical tests (tensile, three-point flexural, compression) were used to study the mechanical properties of materials. Scaffolds with different surfaces were 3D printed from prepared filaments to determine the most optimal surface for cell proliferation. Optical contact angle measurement was conducted to determine the surface properties and their influence on cell adhesion, followed by the calculation of surface free energy. 3D printed surfaces were also subjected to roughness analysis by confocal microscopy to determine the effect of roughness on contact angle with water and cell growth. Finally, in vitro tests on scaffolds were conducted to classify the cytotoxicity of the materials and the influence of the scaffold ́s surface on cell growth and proliferation.

3D printing; FDM; poly(3-hydroxybutyrate); bone tissue engineering; scaffold; biocompatibility; polycaprolactone; polylactic acid

KROBOT, Š.; MELČOVÁ, V.; PŘIKRYL, R.

17. 4. 2023

Czech Chemical Society Symposium

2336-7210

Czech Chemical Society Symposium Series

Česká republika

91

91

122

http://www.ccsss.cz/index.php/ccsss/issue/view/38/69