Enzybiotic-loaded hydrogel dressings for the treatment of infected wounds: a comprehensive study

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angličtina

Wound dressings containing collagen are biologically active, which, in addition to the absorption effect, stimulate the natural activity of cells and angiogenesis. Like collagen, the dressings made of cellulose affect the biochemistry of the wound. E.g., the oxidized cellulose fibers themselves form a cohesive gel in contact with the wound bed, absorbing exudates into the fiber structure and creating a moist environment. The combination of cellulose fibers with collagen thus enables the preparation of materials with improved properties and favorable production costs. Antimicrobial dressings mainly use silver ions and nanoparticles, iodine compounds, chlorhexidine, or antibiotics, the excess of which leads to the resistance of many strains. Thanks to this, recently bacteriophages, isolated antibacterial proteins, enzymes, or peptides have come to the fore. In our project, we prepared both porous lyophilized dressings as well as fully resorbable and easily injectable hydrogels based on the collagen/cellulose biopolymers with incorporated enzybiotics, such as recombinant lysostaphin and endolysin. These hydrogels were subjected to the in vitro method to determine their antibacterial activity on different bacterial strains. The hydrogels themselves are not antibacterial, however, the addition of a low concentration of enzybiotics was sufficient for the high effectiveness of all test hydrogels against specific bacterial strains. The selected hydrogels were then successfully applied to a pig model in skin defects infected with different bacterial strains. Hydrogels can be easily applied even to a complicated and irregular defect. It is also possible to precisely dose enzybiotics to achieve targeted efficacy in complicated infected wounds. Acknowledgment: The study was supported by the Ministry of Health of the Czech Republic, grant No. NV19-05-00214. All rights reserved.

Enzybiotic; hydrogel dressings; infected wounds

VOJTOVÁ, L.; KACVINSKÁ, K.; DORAZILOVÁ, J.; KOBZOVÁ, Š.; VACEK, L.; LIPOVÝ, B.; RAŠKA, F.; JEKLOVÁ, E.; JANDA, L.

17. 10. 2022

Los Angeles, CA, USA

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