Detail publikace

Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry

JUGL, A. PEKAŘ, M.

Originální název

Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed.

Klíčová slova

Hyaluronan; Cecropin B; Interaction; High-resolution ultrasonic spectroscopy; Isothermal titration calorimetry

Autoři

JUGL, A.; PEKAŘ, M.

Vydáno

1. 2. 2023

Nakladatel

ELSEVIER

Místo

AMSTERDAM

ISSN

0141-8130

Periodikum

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ročník

227

Číslo

1

Stát

Nizozemsko

Strany od

786

Strany do

794

Strany počet

9

URL

https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub

BibTex

@article{BUT180491,
  author="Adam {Jugl} and Miloslav {Pekař}",
  title="Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2023",
  volume="227",
  number="1",
  pages="786--794",
  doi="10.1016/j.ijbiomac.2022.12.144",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub"
}