Detail publikace

Hydrogel dressings for phage therapy of chronic wounds

ČERNÁ, E. VOJTOVÁ, L. VACEK, L. LIPOVÝ, B. RŮŽIČKA, F. BENEŠÍK, M. PANTŮČEK, R. BOTKA, T.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Chronic wounds affect about 1-2% of the developed countries population, thereby becoming a worldwide financial threat to the healthcare system, significantly affecting the patients' lifestyle. Chronic wounds tend to have a slow healing process; they can be easily infected with a biofilm-forming multidrug-resistant bacteria. To overcome such a burden, bacteriophages (phages) therapy can combat resistant bacterial infections as an alternative to conventional antibiotics, infecting a specific bacterial strain and leading to their destruction without affecting eucaryotic cells. For effective topical phage application, hydrogels can be used to ensure phage delivery, stability, and controlled release in the wound site. This work describes hydrogel preparation and modification with phages via a freeze-drying technique of polymer blend composed of natural polysaccharide gum Karaya (GK) with synthetic polymers acting as surfactants. Physicochemical characterization shows that the ratio between GK/PVA strongly influences visual appearance, water swelling (60-70% of original weight), retention, and degradation (stable after 28 days, weight loss of 55%). Then the polyvalent phage JK2(=812K1/420) was coated on the surface of the hydrogel. This phage was used as it interacts specifically with Staphylococcus aureus family stains that are frequently present in chronic wounds. The release from hydrogel coated with phages was tested by double agar method with over 90% of released phages, and the antimicrobial activity against Staphylococcus aureus ATCC 43300 (CCM 4750) proved to be effective in 12 hours. Gum Karaya-based hydrogel coated with phages shows the potential to become a suitable carrier for the phage therapy of chronic wounds based on antibacterial tests against strains of Staphylococcus aureus.

Klíčová slova

Hydrogel, chronic wound, phage, phage therapy, wound healing, antimicrobial

Autoři

ČERNÁ, E.; VOJTOVÁ, L.; VACEK, L.; LIPOVÝ, B.; RŮŽIČKA, F.; BENEŠÍK, M.; PANTŮČEK, R.; BOTKA, T.

Vydáno

30. 8. 2022

Nakladatel

Material Research Society of Serbia

Místo

Belgrade, Serbia

ISBN

987-86-919111-7-1

Kniha

TWENTY-THIRD ANNUAL CONFERENCE YUCOMAT 2022 AND TWELFTH WORLD ROUND TABLE CONFERENCE ON SINTERING XII WRTCS

Strany od

Strany do

Strany počet

BibTex

@misc{BUT179008,
  author="Eva {Černá} and Lucy {Vojtová} and Lukáš {Vacek} and Břetislav {Lipový} and Filip {Růžička} and Martin {Benešík} and Roman {Pantůček} and Tibor {Botka}",
  title="Hydrogel dressings for phage therapy of chronic wounds",
  booktitle="TWENTY-THIRD ANNUAL CONFERENCE YUCOMAT 2022 AND TWELFTH WORLD ROUND TABLE CONFERENCE ON SINTERING XII WRTCS",
  year="2022",
  pages="99--99",
  publisher="Material Research Society of Serbia",
  address="Belgrade, Serbia",
  isbn="987-86-919111-7-1",
  note="abstract"
}