Detail publikace

Capture of DNAs by magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres

TÓTH, J. ŠÁLEK, P. GYENIS, J. RITTICH, B.

Originální název

Capture of DNAs by magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres (mgt.HPS1-NH2 and mgt.HPS2-NH2) containing different contents of amino groups were prepared and characterized in this study. The microspheres were used for the capture of uncompacted and compacted bacterial and calf thymus DNAs in the presence of different PEG 6000 and NaCl concentrations. Magnetic macroporous poly(glycidyl methacrylate-co-[2-(methacryloyloxy)ethoxy]acetic acid-co-ethylene dimethacrylate) microspheres containing amino and carboxyl groups [mgt.P(GMA-MOEAA-EDMA)-NH2] and magnetic non-porous carboxyl group-functionalized poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [mgt.P(HEMA-GMA)-COOH] microspheres were used as a control. The size changes of uncompacted and compacted bacterial and calf thymus DNAs were measured in the range of concentrations 0-16% PEG 6000 and 0.5-2.0 M NaCl by dynamic light scattering (DLS). The highest capture of compacted bacterial and calf thymus DNAs was achieved with mgt.HPS1-NH2, mgt.HPS2-NH2 microspheres using 8% PEG 6000 and 2.0 M NaCl and with mgt.P(GMA-MOEAA-EDMA)-NH2 microspheres using 8% and 16% PEG 6000 and 2.0 M NaCl. Developed microspheres were used for DNA isolation from real vegetable (broccoli flowering head) samples. DNA was in quality suitable for PCR.

Klíčová slova

DNA capture

Autoři

TÓTH, J.; ŠÁLEK, P.; GYENIS, J.; RITTICH, B.

Vydáno

3. 1. 2021

Nakladatel

SPRINGER

Místo

NEW YORK

ISSN

0022-2461

Periodikum

Journal of Materials Science

Ročník

56

Číslo

9

Stát

Spojené státy americké

Strany od

5817

Strany do

5829

Strany počet

13

URL

https://doi.org/10.1007/s10853-020-05649-5

BibTex

@article{BUT177299,
  author="Judit {Tóth} and Petr {Šálek} and János {Gyenis} and Bohuslav {Rittich}",
  title="Capture of DNAs by magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres",
  journal="Journal of Materials Science",
  year="2021",
  volume="56",
  number="9",
  pages="5817--5829",
  doi="10.1007/s10853-020-05649-5",
  issn="0022-2461",
  url="https://doi.org/10.1007/s10853-020-05649-5"
}