Detail publikace

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

MRÁZOVÁ, K. NOVÁČKOVÁ, I. KOUŘILOVÁ, X. OBRUČA, S. KRZYŽÁNEK, V.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.

Klíčová slova

PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans

Autoři

MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.

Vydáno

13. 9. 2021

Strany od

Strany do

Strany počet

BibTex

@misc{BUT175387,
  author="Kateřina {Mrázová} and Ivana {Nováčková} and Xenie {Kouřilová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
  title="The first insight on changes in PHA granules after extraction from extremophilic microorganisms",
  booktitle="Microscopy 2021: Conference",
  year="2021",
  series="1",
  edition="1",
  pages="81--81",
  note="abstract"
}