Detail publikace

Biomechanical, structural and in vitro evaluation of a modified biopolymer-based scaffolds for skin tissue engineering applications

KACVINSKÁ, K. PAVLIŇÁKOVÁ, V. POLÁČEK, P. BLAHNOVÁ, V. FILOVA, E. VOJTOVÁ, L.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Introduction: The addition of polysaccharides and bioactive substances in protein-based scaffolds has shown some perspective future views for skin wound applications affecting both antibacterial and healing properties. Materials and Methods: In the presented study, the effect of bioresorbable polysaccharides and bioactive coating on the biomechanical, structural and in-vitro properties of protein-based scaffolds was evaluated. Method of freeze-drying was carried out to produce final skin grafts. Results and Discussion: Due to the ionic interactions with proteins, polysaccharides influence the swelling capacity, porosity and biomechanical properties of protein scaffolding material considering it as desirable and beneficial for restoring the function of the skin. In general, the addition of polysaccharides and bioactive coating of protein-based porous scaffolds had increased strain without any detectable deformation during biomechanical test, which is referred to the improved elasticity of microfibrils. In-vitro biocompatibility assay on mouse fibroblasts 3T3 reported an enhancement in cell adhesion and proliferation, together with a high proportion of viable cells maintained for a significantly longer time throughout the whole culture period of four weeks. Conclusions: Grafts for treating skin defects requires a right balance between elasticity and stiffness, together with soft porous architecture allowing cells to infiltrate, adhere and proliferate. The polysaccharide-protein skin grafts are ideal to meet these requirements, therefore serve as good skin engineered substitutes. Moreover, we found that by the amount and type of polysaccharide and bio-coating, the enzymatic degradation of protein-based scaffolds (its “life-time”) can be tailored in order to follow the rate of skin regeneration.

Klíčová slova

polysaccharide, protein, skin tissue engineering, fibroblasts, scaffold

Autoři

KACVINSKÁ, K.; PAVLIŇÁKOVÁ, V.; POLÁČEK, P.; BLAHNOVÁ, V.; FILOVA, E.; VOJTOVÁ, L.

Vydáno

7. 10. 2021

Nakladatel

České vysoké učení technické v Praze

Místo

Praha

ISBN

978-80-01-06886-1

Kniha

XII. mezinárodní konference „Bioimplantologie 2021“

Edice

České vysoké učení technické v Praze

Číslo edice

Strany od

Strany do

Strany počet

URL

http://www.bioimplantologie.cz/down/bioimplantologie_2021_prg.pdf

BibTex

@misc{BUT175332,
  author="Katarína {Verčimáková} and Veronika {Pavliňáková} and Petr {Poláček} and Veronika {Blahnová} and Eva {Filova} and Lucy {Vojtová}",
  title="Biomechanical, structural and in vitro evaluation of a modified biopolymer-based scaffolds for skin tissue engineering applications",
  booktitle="XII. mezinárodní konference „Bioimplantologie 2021“",
  year="2021",
  series="České vysoké učení technické v Praze",
  edition="1.",
  pages="29--29",
  publisher="České vysoké učení technické v Praze",
  address="Praha",
  isbn="978-80-01-06886-1",
  url="http://www.bioimplantologie.cz/down/bioimplantologie_2021_prg.pdf",
  note="abstract"
}