Detail publikace

Evaluation of Recombinant Kpkt Cytotoxicity on HaCaT Cells: Further Steps towards the Biotechnological Exploitation Yeast Killer Toxins

CARBONI, G. MÁROVÁ, I.

Originální název

Evaluation of Recombinant Kpkt Cytotoxicity on HaCaT Cells: Further Steps towards the Biotechnological Exploitation Yeast Killer Toxins

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The soil yeast Tetrapisispora phaffii secretes a killer toxin, named Kpkt, that shows beta-glucanase activity and is lethal to wine spoilage yeasts belonging to Kloeckera/Hanseniaspora, Saccharomycodes and Zygosaccharomyces. When expressed in Komagataella phaffii, recombinant Kpkt displays a wider spectrum of action as compared to its native counterpart, being active on a vast array of wine yeasts and food-related bacteria. Here, to gather information on recombinant Kpkt cytotoxicity, lyophilized preparations of this toxin (LrKpkt) were obtained and tested on immortalized human keratinocyte HaCaT cells, a model for the stratified squamous epithelium of the oral cavity and esophagus. LrKpkt proved harmless to HaCaT cells at concentrations up to 36 AU/mL, which are largely above those required to kill food-related yeasts and bacteria in vitro (0.25-2 AU/mL). At higher concentrations, it showed a dose dependent effect that was comparable to that of the negative control and therefore could be ascribed to compounds, other than the toxin, occurring in the lyophilized preparations. Considering the dearth of studies regarding the effects of yeast killer toxins on human cell lines, these results represent a first mandatory step towards the evaluation the possible risks associated to human intake. Moreover, in accordance with that observed on Ceratitis capitata and Musca domestica, they support the lack of toxicity of this toxin on non-target eukaryotic models and corroborate the possible exploitation of killer toxins as natural antimicrobials in the food and beverages industries.

Klíčová slova

Kpkt; yeast killer toxin; natural antimicrobial; HaCaT cells

Autoři

CARBONI, G.; MÁROVÁ, I.

Vydáno

2. 3. 2021

Nakladatel

MDPI

Místo

BASEL

ISSN

2304-8158

Periodikum

Foods

Ročník

10

Číslo

3

Stát

Švýcarská konfederace

Strany od

1

Strany do

11

Strany počet

11

URL

http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=E3t3PwZwXLt1SuGZEat&page=1&doc=2

BibTex

@article{BUT171710,
  author="Gavino {Carboni} and Ivana {Márová}",
  title="Evaluation of Recombinant Kpkt Cytotoxicity on HaCaT Cells: Further Steps towards the Biotechnological Exploitation Yeast Killer Toxins",
  journal="Foods",
  year="2021",
  volume="10",
  number="3",
  pages="1--11",
  doi="10.3390/foods10030556",
  issn="2304-8158",
  url="http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=E3t3PwZwXLt1SuGZEat&page=1&doc=2"
}