Detail publikace

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Zdeněk Pilát1*, Alexandr Jonáš1, Jana Pilátová2, Tereza Klementová1, Silvie Bernatová1, Martin Šiler1, Tadeáš Maňka1, Martin Kizovský1, Filip Růžička3, Roman Pantůček4, Ute Neugebauer5,6, Ota Samek1, Pavel Zemánek

Originální název

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Bacteriophages, or "phages" for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.

Klíčová slova

STAPHYLOCOCCUS-AUREUS; ELECTRON-TRANSPORT; VIRUS STRUCTURE; SPECTROSCOPY; PHAGE; INHIBITION; TRANSCRIPTION; EXPRESSION; CYTOCHROME; CAPSIDS

Autoři

Zdeněk Pilát1*, Alexandr Jonáš1, Jana Pilátová2, Tereza Klementová1, Silvie Bernatová1, Martin Šiler1, Tadeáš Maňka1, Martin Kizovský1, Filip Růžička3, Roman Pantůček4, Ute Neugebauer5,6, Ota Samek1, Pavel Zemánek

Vydáno

20. 8. 2020

Nakladatel

AMER CHEMICAL SOC

Místo

WASHINGTON

ISSN

1520-6882

Periodikum

Analytical Chemistry

Ročník

92

Číslo

18

Stát

Spojené státy americké

Strany od

12304

Strany do

12311

Strany počet

8

URL

https://pubs.acs.org/doi/10.1021/acs.analchem.0c01963

BibTex

@article{BUT165603,
  author="Zdeněk {Pilát} and Tereza {Klementová}",
  title="Analysis of Bacteriophage-Host Interaction by Raman Tweezers",
  journal="Analytical Chemistry",
  year="2020",
  volume="92",
  number="18",
  pages="12304--12311",
  doi="10.1021/acs.analchem.0c01963",
  issn="1520-6882",
  url="https://pubs.acs.org/doi/10.1021/acs.analchem.0c01963"
}