Detail publikace

The effect of healing phenotype-inducing cytokine formulations within soft hydrogels on encapsulated monocytes and incoming immune cells

Ivana Ščigalková, Julie Bystroňová, Lenka Kovářová, Martin Pravda, Vladimír Velebný, Vladimir Riabov, Harald Klüter, Julia Kzhyshkowska, Nihal Engin Vrana

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The adverse immune responses to implantable biomedical devices is a general problem with important consequences for the functionality of implants. Immunomodulatory soft hydrogel-based interfaces between the implant and the host can attenuate these reactions. Moreover, encapsulation of the patient's own immune cells into these interfaces can lead to the personalisation of implants from the immune reaction point of view. Herein, we described a co-crosslinkable composite hydrogel (composed of gelatin and hyaluronic acid), which could be used for the encapsulation of macrophages in the presence of an anti-inflammatory phenotype-fixing cytokine cocktail. To mimick the incoming immune cells on the coating surface in vivo, peripheral blood mononuclear cells were seeded on the hydrogels. The encapsulation of monocytic cells into the composite hydrogels in the presence of cytokine cocktails at 5x or 10x concentrations led to the spreading of the encapsulated cells instead of the formation of clusters. Moreover, the secretion of the anti-inflammatory cytokines IL-1RA and CCL-18 was significantly increased. The attachment of PBMC to the surface of the hydrogel is dependent on the hydrogel composition and also significantly increased in the presence of the cytokine cocktail together with the number of CD68+ cells on the hydrogel surface. Our study demonstrates that the delivery of a polarisation cocktail with biocompatible hydrogels can control the initial response by the incoming immune cells. This effect can be improved by the encapsulation of autologous monocytes that are also polarised by the cytokine cocktail and secrete additional anti-inflammatory cytokines. This interface can fine tune the initial immune response to an implanted biomaterial in a personalised manner.

Klíčová slova

hydrogels; cells; cytokine; encapsulation

Autoři

Ivana Ščigalková, Julie Bystroňová, Lenka Kovářová, Martin Pravda, Vladimír Velebný, Vladimir Riabov, Harald Klüter, Julia Kzhyshkowska, Nihal Engin Vrana

Vydáno

10. 6. 2019

Nakladatel

ROYAL SOC CHEMISTRY

Místo

CAMBRIDGE

ISSN

2046-2069

Periodikum

RSC Advances

Ročník

Číslo

Stát

Spojené království Velké Británie a Severního Irska

Strany od

21396

Strany do

21404

Strany počet

URL

https://doi.org/10.1039/C9RA02878A

BibTex

@article{BUT163639,
  author="Ivana {Ščigalková} and Lenka {Kovářová}",
  title="The effect of healing phenotype-inducing cytokine formulations within soft hydrogels on encapsulated monocytes and incoming immune cells",
  journal="RSC Advances",
  year="2019",
  volume="9",
  number="37",
  pages="21396--21404",
  doi="10.1039/c9ra02878a",
  issn="2046-2069",
  url="https://doi.org/10.1039/C9RA02878A"
}