Detail publikace

New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures

PUENTE-MUNOZ, V. PAREDES, J., M. Resa, S. Vilchez, J., D. Žitňan, M. Miguel, D. Giron, M., D. Cuerva, J., M. Salto, R. Crovetto, L.

Originální název

New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the levels of biothiols associated with ROS generation via single-cell bioimaging.

Klíčová slova

molecular biology, chemistry

Autoři

PUENTE-MUNOZ, V.; PAREDES, J., M.; Resa, S.; Vilchez, J., D.; Žitňan, M.; Miguel, D.; Giron, M., D.; Cuerva, J., M.; Salto, R.; Crovetto, L.

Vydáno

7. 2. 2019

Nakladatel

NATURE PUBLISHING GROUP

Místo

LONDON

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

9

Číslo

2019

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1659

Strany do

1669

Strany počet

10

URL

https://www.nature.com/articles/s41598-018-38132-y

BibTex

@article{BUT161966,
  author="PUENTE-MUNOZ, V. and PAREDES, J., M. and Resa, S. and Vilchez, J., D. and Žitňan, M. and Miguel, D. and Giron, M., D. and Cuerva, J., M. and Salto, R. and Crovetto, L.",
  title="New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures",
  journal="Scientific Reports",
  year="2019",
  volume="9",
  number="2019",
  pages="1659--1669",
  doi="10.1038/s41598-018-38132-y",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-018-38132-y"
}