Adaptation of PHA producing bacteria Cupriavidus necator H16 and Halomonas halophila to biotechnologically relevant stressors

abstrakt

angličtina

Selected microorganisms Cupriavidus necator H16 (CCM 3726) and Halomonas halophila (CCM 3662) have been exposed to several biotechnologically relevant stressors. C. necator H16 was exposed to presence of NaCl causing osmotic stress which represented environmental stress factor and to copper representing anthropogenic pollutant. Strain H. halophila was exposed to acetic and levulinic acid; components of hydrolysate of lignocellulosic biomass. Evolutionary experiments were provided using multiple serial transfers of cell cultures in Erlenmeyer flasks, always after 48 hours of cultivation. Basic screening accompanying every step included determination of optical density of cell cultures, gravimetrical determination of dry cell weight, GC-FID analysis of PHAs in biomass and also determination of organic acids in supernatants of H. halophila. After more than 20 and 40 transfers, cultures were preserved for further characterization. Potential of PHAs accumulation, effectivity of utilization of organic acids, testing of robustness, changes in cell morphology, physico-chemical properties of cells and also changes in genome and transcriptome were determined within comparison of evolved strains with wild-type ones.

evolutionary engineering; polyhydroxyalkanoates; C. necator H16, H. halophila

NOVÁČKOVÁ, I.; KUČERA, D.; OBRUČA, S.; SEDLÁČEK, P.; POŘÍZKA, J.; PERNICOVÁ, I.

25. 9. 2019

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