Detail publikace

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

VANĚK, M. MRAVEC, F. SZOTKOWSKI, M. BYRTUSOVÁ, D. HÁRONIKOVÁ, A. MÁROVÁ, I.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.

Klíčová slova

autofluorescence, carotenoids, Cystofilobasidium capitatum, fluorescence lifetime imaging, red yeasts

Autoři

VANĚK, M.; MRAVEC, F.; SZOTKOWSKI, M.; BYRTUSOVÁ, D.; HÁRONIKOVÁ, A.; MÁROVÁ, I.

Vydáno

10. 4. 2018

Nakladatel

De Gruyter Open

Místo

Itálie

ISSN

2564-615X

Periodikum

The EuroBiotech Journal

Ročník

Číslo

Stát

Italská republika

Strany od

114

Strany do

120

Strany počet

URL

http://www.degruyter.com/view/j/ebtj.2018.2.issue-2/ebtj-2018-0015/ebtj-2018-0015.xml?format=INT

BibTex

@article{BUT155466,
  author="Martin {Vaněk} and Filip {Mravec} and Martin {Szotkowski} and Dana {Byrtusová} and Andrea {Němcová} and Ivana {Márová}",
  title="Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth",
  journal="The EuroBiotech Journal",
  year="2018",
  volume="2",
  number="2",
  pages="114--120",
  doi="10.2478/ebtj-2018-0015",
  issn="2564-615X",
  url="http://www.degruyter.com/view/j/ebtj.2018.2.issue-2/ebtj-2018-0015/ebtj-2018-0015.xml?format=INT"
}

Dokumenty

[2564615X - The EuroBiotech Journal] Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth.pdf