Detail publikace

Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila

KUČERA, D. PERNICOVÁ, I. KOVALČÍK, A. KOLLER, M. MÜLLEROVÁ, L. SEDLÁČEK, P. MRAVEC, F. NEBESAROVA, J. KALINA, M. MÁROVÁ, I. KRZYŽÁNEK, V. OBRUČA, S.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer’s molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl; required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production.

Klíčová slova

Halomonas halophila; Halophiles; Lignocellulose hydrolysates; Morphology of bacterial cells; Polyhydroxyalkanoates

Autoři

KUČERA, D.; PERNICOVÁ, I.; KOVALČÍK, A.; KOLLER, M.; MÜLLEROVÁ, L.; SEDLÁČEK, P.; MRAVEC, F.; NEBESAROVA, J.; KALINA, M.; MÁROVÁ, I.; KRZYŽÁNEK, V.; OBRUČA, S.

Vydáno

1. 5. 2018

Nakladatel

Elsevier

ISSN

0960-8524

Periodikum

BIORESOURCE TECHNOLOGY

Ročník

256

Číslo

Stát

Nizozemsko

Strany od

552

Strany do

556

Strany počet

URL

https://www.sciencedirect.com/science/article/pii/S0960852418302554

BibTex

@article{BUT147942,
  author="KUČERA, D. and PERNICOVÁ, I. and KOVALČÍK, A. and KOLLER, M. and MÜLLEROVÁ, L. and SEDLÁČEK, P. and MRAVEC, F. and NEBESAROVA, J. and KALINA, M. and MÁROVÁ, I. and KRZYŽÁNEK, V. and OBRUČA, S.",
  title="Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila",
  journal="BIORESOURCE TECHNOLOGY",
  year="2018",
  volume="256",
  number="1",
  pages="552--556",
  doi="10.1016/j.biortech.2018.02.062",
  issn="0960-8524",
  url="https://www.sciencedirect.com/science/article/pii/S0960852418302554"
}