Detail publikace

High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group

KONEČNÁ, J. CHVALKOVSKÁ, E. KNÁPKOVÁ, M. ŠPANOVÁ, A. RITTICH, B. BRÁZDA, V.

Originální název

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.

Klíčová slova

Lactobacillus casei; DNA isolation; High-Resolution Melting Analysis

Autoři

KONEČNÁ, J.; CHVALKOVSKÁ, E.; KNÁPKOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.; BRÁZDA, V.

Vydáno

25. 4. 2018

ISBN

978-80-223-4517-0

Kniha

Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov

Číslo edice

první

Strany od

302

Strany do

307

Strany počet

1652

BibTex

@inproceedings{BUT147532,
  author="Jana {Konečná} and Eva {Chvalkovská} and Monika {Knápková} and Alena {Španová} and Bohuslav {Rittich} and Václav {Brázda}",
  title="High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group",
  booktitle="Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov",
  year="2018",
  number="první",
  pages="302--307",
  isbn="978-80-223-4517-0"
}