Advanced Microrheological Techniques In The Research Of Hydrogels

abstrakt

angličtina

This work is focused on the use of advanced fluorescence correlation spectroscopy technique (FCS) in microrheological characterization of hydrogels. Hydrogels were prepared from negatively charged polymer (sodium hyaluronate) and oppositely charged surfactant (cetyltrimethylammonium bromide) in physiological environment. Low, medium and high molecular weight sodium hyaluronate was used. Our hydrogels contain more than 90 % of water and they are able to solubilize hydrophobic and hydrophilic species. These hydrogels contain hydrophobic nanodomains (nanocontainers) and can be promising materials for the use in many applications, e.g. in topical applications or local drug delivery systems. Main aim of this work is to study microrhelological behavior of these systems and usability of FCS technique in these systems. For microrheological FCS analysis were used fluorescently labelled particles. Optimum particle size was selected based on analysis of Newtonian glycerol solutions. The results of FCS hydrogel microrheology were compared with the measurement of hyaluronan solutions. Concentration and molecular weight of this biopolymer were monitored.

hydrogels, fluorescence correlation spectroscopy (FCS), nanocontainers, surfactants

JARÁBKOVÁ, S.; KÁBRTOVÁ, P.; MRAVEC, F.; PEKAŘ, M.

10. 9. 2017

Johan Hofkens, Maarten Roeffaers & Kris Janssen

Bruggy, Belgie

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