Detection of Lactobacillus acidophilus species in the probiotic meat product

abstrakt

angličtina

Probiotic bacteria have beneficial effects on human health. They positively influence the host immune system and the composition of intestinal microflora. They can reach the gastrointestinal tract with food. Probiotic bacteria are found in dairy products and food supplements most often. They can be used in other matrices including meat products. The aim of this study was to detect Lactobacillus acidophilus cells in probiotic fermented meat product “Ovčácká klobása” using the PCR method. Attention was paid to the preparation of the sample (homogenization, lysis of cells) and optimization of methods for the isolation of DNA in quality suitable for PCR using magnetic microparticles. The use of magnetic particles with immobilized antibody antiLactobacillus was also tested for Lactobacillius acidophilus bacterial cells isolation from homogenized product. Bacterial cells bound on the magnetic particles were identified using PCR. Specific PCR products for the species Lactobacillus acidophilus were detected in both cases. In the control fermented meat product “Orlík”, which had not been declared to contain L. acidophilus, the species was not detected. It was demonstrated that immunomagnetic separation can be used for the isolation and concentration of cells.

probiotic meat products, Lactobacillus, immunomagnetic separation

VAŠÍČEK, R.; VAŇÁSEK, J.; KONEČNÁ, J.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

2. 9. 2015

Brno University of Technology, Faculty of Chemistry

Brno, Czech Republic

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