Study of nanoparticles formed by cationic micelles and hyaluronan

článek ve sborníku ve WoS nebo Scopus

angličtina

Hyaluronan as negatively charged polyelectrolyte can interact with positively charged surfactant micelles via electrostatic interactions to form core-shell like nanoparticles. These aggregates can solubilize hydrophobic active substances and therefore are potential carriers in drug delivery applications. This research was conducted with the aim to prepare nanoparticles consisting of hyaluronan and cationic micelles and evaluate strength of electrostatic interactions between components. Interactions of hyaluronan and surfactants in aqueous solution were investigated using turbidimetry method and stability of nanoparticles was studied using dialysis technique. Turbidimetric titration was chosen as indicator of the loss of intensity of transmitted light due to the scattering effect of particles associated from hyaluronan and surfactant in it. Results of turbidimetry revealed that aggregates formation depends on hyaluronan concentration while surfactant concentration (above critical micelle concentration) affects interaction insignificantly. On the basis of turbidimetric results a system for dialysis experiments was selected. Dialysis experiments showed that a part of surfactant molecules is bound to hyaluronan chain and a rest of molecules diffuse to water through dialysis membrane and after a lapse of time the concentrations of the retentate and permeate were balanced. The system can be suitable for the preparation of targeted carriers of biologically active substances.

hyaluronan, surfactant, nanoparticles, turbidimetry, dialysis

PILGROVÁ, T.; PEKAŘ, M.

2015

14. 10. 2015

TANGER Ltd

Ostrava

978-80-87294-63-5

NANOCON 2015 Conference Proceedings

404

407

4

http://www.nanocon.eu/files/proceedings/23/papers/4232.pdf