Detail publikace
Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion.
ČOLLÁKOVÁ, J. KŘÍŽOVÁ, A. KOLLÁROVÁ, V. DOSTÁL, Z. SLABÁ, M. VESELÝ, P. CHMELÍK, R.
Originální název
Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion.
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Coherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by cytopathic turbid emulsions. CCHM capacity was first assessed in model situations that showed clear images obtained with low coherence of illumination but not with high coherence of illumination. Then, the form of death of human cancer cells induced by treatment with biologically active phospholipids (BAPs) preparation was investigated. The observed overall retraction of cell colony was apparently caused by the release of cell-to-substratum contacts. This was followed by the accumulation of granules decorating the nuclear membrane. Then, the occurrence of nuclear membrane indentations signaled the start of damage to the integrity of the cell nucleus. In the final stage, cells shrunk and disintegrated. This indicated that BAPs cause cell death by necrosis and not apoptosis. An intriguing option of checking the fate of cancer cells caused by the anticipated cooperative effect after adding another tested substance sodium dichloroacetate to turbid emulsion is discussed on grounds of pilot experiments. Such observations should reveal the impact and mechanism of action of the interacting drugs on cell behavior and fate that would otherwise remain hidden in turbid milieu.
Klíčová slova
quantitative phase imaging; holographic microscopy; imaging through turbid media; coherence gate effect; live cell imaging, cancer cell necrotic death
Autoři
ČOLLÁKOVÁ, J.; KŘÍŽOVÁ, A.; KOLLÁROVÁ, V.; DOSTÁL, Z.; SLABÁ, M.; VESELÝ, P.; CHMELÍK, R.
Rok RIV
2015
Vydáno
1. 11. 2015
Nakladatel
Journal of Biomedical Optics SPIE
ISSN
1083-3668
Periodikum
JOURNAL OF BIOMEDICAL OPTICS
Ročník
20
Číslo
11
Stát
Spojené státy americké
Strany od
111213-1
Strany do
111213-7
Strany počet
7
URL
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