Detail publikace

Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles

KUDR, J. NEJDL, L. SKALIČKOVÁ, S. ŽŮREK, M. MILOSAVLJEVIĆ, V. KENŠOVÁ, R. RUTTKAY-NEDECKÝ, B. KOPEL, P. HYNEK, D. NOVOTNÁ, M. ADAM, V. KIZEK, R.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface. The thermal treatment of apoferritin with CdTe NPs resulted in the aggregation of cores, which was indicated by changes in the absorption spectra and the shape of apoferritin tryptophan fluorescence. The apoferritin modified with CdTe NPs was additionally modified with gold nanoparticles and attached to magnetic particles via oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.

Klíčová slova

MODIFIED MAGNETIC PARTICLES; HORSE SPLEEN APOFERRITIN; BOVINE SERUM-ALBUMIN; CAGE-SHAPED PROTEIN; CDTE QUANTUM DOTS; GOLD NANOPARTICLES; BIOMIMETIC SYNTHESIS; THERMAL-STABILITY; CDS NANOPARTICLES; AQUEOUS-SOLUTIONS

Autoři

KUDR, J.; NEJDL, L.; SKALIČKOVÁ, S.; ŽŮREK, M.; MILOSAVLJEVIĆ, V.; KENŠOVÁ, R.; RUTTKAY-NEDECKÝ, B.; KOPEL, P.; HYNEK, D.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Rok RIV

2015

Vydáno

14. 3. 2015

ISSN

2050-750X

Periodikum

Journal of Materials Chemistry B

Ročník

Číslo

Stát

Spojené království Velké Británie a Severního Irska

Strany od

2109

Strany do

2118

Strany počet

BibTex

@article{BUT113985,
  author="Jiří {Kudr} and Lukáš {Nejdl} and Sylvie {Skaličková} and Michal {Žůrek} and Vedran {Milosavljević} and Renáta {Kenšová} and Branislav {Ruttkay-Nedecký} and Pavel {Kopel} and David {Hynek} and Marie {Novotná} and Vojtěch {Adam} and René {Kizek}",
  title="Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles",
  journal="Journal of Materials Chemistry B",
  year="2015",
  volume="3",
  number="10",
  pages="2109--2118",
  doi="10.1039/c4tb01336k",
  issn="2050-750X"
}