Detail publikace

Spectroscopic and Structural Analysis of Humic Substances Isolated from Forest Soil Humic Podzol - Locality Krkonoše, Czech Republic

ENEV, V. KLUČÁKOVÁ, M. NOVÁK, F.

Originální název

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

The aim of this work was study chemical composition, chemical properties and humification degree of different soil humic substances (HS). The object of our study were three various samples of HS. HAs and FA were isolated from forest soil Humic Podzol (locality Krkonoše, Czech Republic). Isolation of soil HS was performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of soil HS were calculated from the absorbance values. Infrared spectroscopy is a useful technique in characterization of structure, functional groups and formation modes of HS. For the fluorescence experiments the final concentration of the HS was adjusted to 10 mg/dm3. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Fluorescence mono-dimensional spectra and total luminescence spectra (TLS) of soil HS were obtained using steady-state fluorescence spectroscopy. All fluorescence spectra were performed on a Horiba Scientific Fluorolog. Fluorescence index (Milori index and HIX) of HS was calculated from the area of the emission spectra. The fluorescence intensity (IF) values (in CPS) of samples were corrected using method of Lakowicz. Soil A55 HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. HS isolated from organic horizon were characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.

Klíčová slova

soil humic and fulvic acids, fluorescence, absorption and fluorescence indexes, chemical composition and structure, humification degree, O-containing functional groups

Autoři

ENEV, V.; KLUČÁKOVÁ, M.; NOVÁK, F.

Rok RIV

2014

Vydáno

4. 12. 2014

Nakladatel

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Místo

Brno

ISBN

978-80-214-5077-6

Kniha

Studentská odborná konference: Chemie je život 2014, Sborník příspěvků

Číslo edice

první

Strany od

216

Strany do

222

Strany počet

BibTex

@inproceedings{BUT111324,
  author="Vojtěch {Enev} and Martina {Klučáková} and František {Novák}",
  title="Spectroscopic and Structural Analysis of Humic Substances Isolated from Forest Soil Humic Podzol - Locality Krkonoše, Czech Republic",
  booktitle="Studentská odborná konference: Chemie je život 2014, Sborník příspěvků",
  year="2014",
  number="první",
  pages="216--222",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  address="Brno",
  isbn="978-80-214-5077-6"
}