Significant bactericidal effects of gelatin nanofibers modified with oxidized cellulose

přednáška

angličtina

This work is focused on the preparation of gelatin nanofibers that are doped with salt of oxidized cellulose. Gelatin is a mixture of proteins which is obtained by partial hydrolysis of natural protein - collagen. Gelatin is a biocompatible, biodegradable material with high absorption capacity. Oxidized cellulose and its salts are also biocompatible, biodegradable and they have antibacterial and hemostatic effects. In this study we developed a new material which combine the properties both of them. Nanofibers with unique bactericide efficiency was obtained via electrospinning process. Chemical composition of the fibers has been studied by ATR-FTIR spectroscopy and EDX elemental analysis. The results of these analysis confirmed the presence of salt of oxidized cellulose in prepared mats. Diameter of nanofibers and their morphology were determined by scanning electron microscopy (SEM). Bactericidal effect was examined by the method of chemical bioluminescence on modified bacterial strain of E. coli. This method proved complete mortality in most of cases. Newly prepared material may have a potential application in tissue engineering and regenerative medicine of soft tissue.

electrospinning, gelatin, oxidized cellulose, bactericidal effects, nanofibers

Švachová Veronika, Pavliňák David, Vojtová Lucy, Hyršl Pavel, Jančář Josef, Libor Vojtek

22. 8. 2014