Detail publikace

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

KALBÁČOVÁ, M. VERDÁNOVÁ, M. MRAVEC, F. HALASOVÁ, T. PEKAŘ, M.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.

Klíčová slova

Cetyltrimethylammonium bromide; Cytoprotection; Cytotoxicity; Hyaluronan; Keratinocyte; Osteoblast

Autoři

KALBÁČOVÁ, M.; VERDÁNOVÁ, M.; MRAVEC, F.; HALASOVÁ, T.; PEKAŘ, M.

Rok RIV

2014

Vydáno

8. 9. 2014

ISSN

0927-7757

Periodikum

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Ročník

460

Číslo

Stát

Nizozemsko

Strany od

204

Strany do

208

Strany počet

BibTex

@article{BUT109678,
  author="KALBÁČOVÁ, M. and VERDÁNOVÁ, M. and MRAVEC, F. and HALASOVÁ, T. and PEKAŘ, M.",
  title="Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types",
  journal="Colloids and Surfaces A: Physicochemical and Engineering Aspects",
  year="2014",
  volume="460",
  number="1",
  pages="204--208",
  issn="0927-7757"
}