Detail publikace

The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes

MICHALICOVÁ, P. BORŮVKOVÁ, T. MRAVEC, F. ČÁSLAVSKÝ, J. PEKAŘ, M.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.

Klíčová slova

hyaluronan, drying, tert-butanol, gas chromatography

Autoři

MICHALICOVÁ, P.; BORŮVKOVÁ, T.; MRAVEC, F.; ČÁSLAVSKÝ, J.; PEKAŘ, M.

Vydáno

7. 9. 2014

Nakladatel

Czech Chemical Society

ISSN

0009-2770

Periodikum

Chemické listy

Ročník

108

Číslo

Stát

Česká republika

Strany od

772

Strany do

772

Strany počet

BibTex

@misc{BUT109297,
  author="Petra {Michalicová} and Tereza {Borůvková} and Filip {Mravec} and Josef {Čáslavský} and Miloslav {Pekař}",
  title="The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes",
  booktitle="Chemické listy",
  year="2014",
  journal="Chemické listy",
  volume="108",
  number="8",
  pages="772--772",
  publisher="Czech Chemical Society",
  issn="0009-2770",
  note="abstract"
}