Ethanol production from waste paper using immobilized Saccharomyces cerevisiae cells

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angličtina

Ethanol is becoming an important alternative fuel nowadays. For its production, there are efforts made to utilize sources that are cheap and do not compete with food. Some lignocellulosic materials, among which waste paper belongs, fulfill these conditions. Paper has to be hydrolyzed to fermentable sugars first. For hydrolysis the intention was to use yeasts with cellulolytic activity. After screening, chosen yeasts were tested for cellulolytic enzymes production but according to results they were not suitable for hydrolysis of paper and therefore commercial enzymatic preparation was used. The hydrolysis and fermentation to ethanol were performed in one step by the method called simultaneous sacharification and fermentation (SSF). For fermentation yeasts Saccharomyces cerevisiae immobilized into the carrier from plyvinylalcohol by technology Lentikat's were used. The aim of the work was to optimize conditions of SSF - the enzyme and substrate dosage, the addition of nutrients - and to verify these optimized conditions in the laboratory fermentor. Optimal enzyme dosage was determined to 3 % w/w for cellulolytic complex and 0.6 % w/w for b-glucosidase. The substrate dosage was determined to 6 % w/v where the conversion yield was the highest (58 %). The addition of nutrients did not have any relevant impact on the ethanol production. The final ethanol concentration after 120 hours of the process in the fermentor was 15.3 g/l. The results were evaluated by measuring the concentration of glucose and ethanol in the medium by HPLC method with refractometric detection.

ethanol, waste paper, Saccharomyces cerevisiae

ZICHOVÁ, M.; BABÁK, L.; ROSENBERG, M.; STRATILOVÁ, E.; OMELKOVÁ, J.

20. 5. 2014

1336-4839

41st ANNUAL CONFERENCE ON YEAST

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