Detail publikace

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

ŠESTÁK, J. KAHLE, V.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.

Klíčová slova

Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters

Autoři

ŠESTÁK, J.; KAHLE, V.

Rok RIV

2014

Vydáno

11. 6. 2014

ISSN

0021-9673

Periodikum

Journal of Chromatography A

Ročník

1350

Číslo

Stát

Nizozemsko

Strany od

Strany do

Strany počet

BibTex

@article{BUT107541,
  author="Jozef {Šesták} and Vladislav {Kahle}",
  title="Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns",
  journal="Journal of Chromatography A",
  year="2014",
  volume="1350",
  number="6",
  pages="68--71",
  issn="0021-9673"
}