Detail publikace

UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed

BOLECHOVÁ, M. ČÁSLAVSKÝ, J. POSPÍCHALOVÁ, M. KOSUBOVÁ, P.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to human and livestock; hence there is a need for methods that allow determination of these dangerous plant toxins. In this study, fast, reliable and sensitive approach is proposed to identify and quantify PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Sample preparation is based on modified QuEChERS approach. Mean recovery, precision, matrix effects and limits of quantification (LOQ) were assessed for three matrices within the method validation. The presented method was tested on 41 various feed samples, where presence of PAs was expected.Roughages and feed for rabbits contained the highest levels of PAs, in general.

Klíčová slova

Pyrrolizidine alkaloids; Feeds; Plant toxins; UPLC-MS/MS; QuEChERS

Autoři

BOLECHOVÁ, M.; ČÁSLAVSKÝ, J.; POSPÍCHALOVÁ, M.; KOSUBOVÁ, P.

Rok RIV

2015

Vydáno

1. 3. 2015

Nakladatel

Elsevier

ISSN

0308-8146

Periodikum

FOOD CHEMISTRY

Ročník

170

Číslo

Stát

Spojené království Velké Británie a Severního Irska

Strany od

265

Strany do

270

Strany počet

BibTex

@article{BUT105580,
  author="BOLECHOVÁ, M. and ČÁSLAVSKÝ, J. and POSPÍCHALOVÁ, M. and KOSUBOVÁ, P.",
  title="UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed",
  journal="FOOD CHEMISTRY",
  year="2015",
  volume="170",
  number="3",
  pages="265--270",
  doi="10.1016/j.foodchem.2014.08.072",
  issn="0308-8146"
}